Journal: BioMed Research International

Article Title: Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits

doi: 10.1155/2017/2089645

Figure Lengend Snippet: Primary and secondary antibodies and normal sera used in the present study.

Article Snippet: Rabbit polyclonal IgG anti-aromatase , 1 : 500 , NB-200-1596 , Novus Biologicals.

Techniques:

Journal: BioMed Research International

Article Title: Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits

doi: 10.1155/2017/2089645

Figure Lengend Snippet: Serum concentrations of total estradiol (E2, (a)) and testosterone (T, (b)) vary between the C, OVX, and OVX + EB groups. (c) The ratio of E2 to T (as logarithm) was calculated to estimate the extent of extragonadal aromatization. Data are the mean ± SEM ( n = 6 per group). (d) Aromatase expression in the ovary (O) and vagina (V) for control rabbits; Ponceau's Red staining was used to corroborate equal amounts of protein were loaded. Paravaginal neurons from the C (e), OVX (f), and OVX + EB (g) groups express aromatase as showed by immunohistochemistry. (h) Percentages of aromatase-ir neurons are means ± SEM ( n = 6 per group). One-way ANOVA followed by Newman–Keuls post hoc tests were carried out to determine significant differences between groups. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 (compared to the C group); & P < 0.001 (compared to the OVX group). −n, negative neurons; +n, positive neurons; +SGC, positive satellite glial cell. Bar, 20 µ m.

Article Snippet: Rabbit polyclonal IgG anti-aromatase , 1 : 500 , NB-200-1596 , Novus Biologicals.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Primary and secondary antisera used for Western blot, IFL, and IHC.

Article Snippet: Aromatase (Cyp19) anti-rabbit polyclonal IgG (E-AB-64300, Elabscience: Houston, TX, USA) , 1:2000.

Techniques: Western Blot, Plasmid Preparation

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Modulation of Kiss1R, Kiss1, Cyp19, GnRH , and miR expression in mediobasal hypothalamus homogenates. Representative Western blots ( n = 4) for Kiss1R, Kiss1, and Cyp19 (A) and normalization of Kiss1R (B) , Kiss1 (C) , and Cyp19 signals (D) carried out against α-tubulin. Quantitative expression ( n = 6) of GnRH (E) and miR-132-3p , Mir-145-5p , let-7a-5p , and let-7b-5p (F–I) carried out by qPCR. Data are expressed as protein OD/tubulin OD ± standard deviation or normalized fold expression (n.f.e.) ± SEM against the housekeeping genes HPRT / β-actin / U6 and the control group used as reference sample. C, control group (placebo injected animals); KP, animals injected with Kp10; AEA, animals injected with AEA; SR + AEA (SA), animals first received SR141716A and 30 min later received AEA treatment. ** p < 0.01, * p < 0.05 vs. the control group.

Article Snippet: Aromatase (Cyp19) anti-rabbit polyclonal IgG (E-AB-64300, Elabscience: Houston, TX, USA) , 1:2000.

Techniques: Expressing, Western Blot, Standard Deviation, Control, Injection

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Modulation of the intratesticular markers of steroidogenesis and GnRH . Representative Western blots for 3βHsd and Cyp19 (A) and normalization of 3βHsd (B) and Cyp19 (C) signals carried out against α-tubulin. Quantitative expression of 3βHsd , Cyp19 , and GnRH mRNA carried out by qPCR (D) . Data are representative of n = 6 different animals and are expressed as protein OD/tubulin OD ± standard deviation or normalized fold expression (n.f.e.) ± SEM against the housekeeping genes HPRT / β-actin and the control group used as a reference sample. C, control group (placebo-injected animals); KP, animals injected with Kp10; * p < 0.05.

Article Snippet: Aromatase (Cyp19) anti-rabbit polyclonal IgG (E-AB-64300, Elabscience: Houston, TX, USA) , 1:2000.

Techniques: Western Blot, Expressing, Standard Deviation, Control, Injection

Journal: PLoS ONE

Article Title: Local Transformations of Androgens into Estradiol by Aromatase P450 Is Involved in the Regulation of Prolactin and the Proliferation of Pituitary Prolactin-Positive Cells

doi: 10.1371/journal.pone.0101403

Figure Lengend Snippet: a) preabsorption and expression in the pituitary, b) positive controls from the ovary of a female adult rat, and from a prolactinoma from a female old rat. Images showing the expression of aromatase in the pituitary of adult male rats. c) Aromatase-positive cells revealed by immunohistochemistry. d) mRNA-aromatase-positive cells detected by in situ hybridization. Negative controls for in situ hybridization: e) absence of the probe and f) sense aromatase probe. Scale bar: c, d: 50 µm.

Article Snippet: The nitrocellulose membranes were then incubated overnight with either preabsorbed anti-aromatase serum or anti rat aromatase P450 rabbit polyclonal serum (Rb-SG 872 Sigma), diluted 1∶500.

Techniques: Expressing, Immunohistochemistry, In Situ Hybridization